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Jackson Laboratory slc1a3 creert females
(A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia <t>using</t> <t>Slc1a3-CreERT</t> driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.
Slc1a3 Creert Females, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slc1a3+creert+females/pmc13016477-307-12-14?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
slc1a3 creert females - by Bioz Stars, 2026-07
86/100 stars

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1) Product Images from "Plagl1 regulates the retinal progenitor cell to Müller glial cell transition"

Article Title: Plagl1 regulates the retinal progenitor cell to Müller glial cell transition

Journal: PLOS Genetics

doi: 10.1371/journal.pgen.1012020

(A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia using Slc1a3-CreERT driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.
Figure Legend Snippet: (A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia using Slc1a3-CreERT driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.

Techniques Used: Transgenic Assay, Gene Expression, Immunostaining, Control



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Jackson Laboratory slc1a3 creert females
(A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia <t>using</t> <t>Slc1a3-CreERT</t> driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.
Slc1a3 Creert Females, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slc1a3+creert+females/pmc13016477-307-12-14?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
slc1a3 creert females - by Bioz Stars, 2026-07
86/100 stars
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(A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia using Slc1a3-CreERT driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.

Journal: PLOS Genetics

Article Title: Plagl1 regulates the retinal progenitor cell to Müller glial cell transition

doi: 10.1371/journal.pgen.1012020

Figure Lengend Snippet: (A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia using Slc1a3-CreERT driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.

Article Snippet: Plagl1 fl/+ males (received from Pengpeng Bi) [ ] were crossed with Slc1a3-CreERT females (JAX:012586) to generate Plagl1 cKOs.

Techniques: Transgenic Assay, Gene Expression, Immunostaining, Control